Biwott crossed the line finish line after 2:10:34 beating world cross country champion Geoffery Kamworor (KEN) and Boston marathon champion Lelisa Desisa of Ethiopia.
Former Boston Marathon champion the 40-year-old Meb Keflezighi was the top American finishing in 7th place in 2:13:32.
"It was fantastic. "The pace was slow (in the first half of the race) but I knew that last 15 kilometres I pushed hard." said the 29-year-old Biwott who split massive 28.35min for the last 10km.
Defending NY marathon champion Wilson Kipsang finished in fourth in 2:12.45.
Last year's champion Mary Keitany pushed away from the lead pack at 29km marker to win her second NY marathon title with a time of 2:24:25.
Keitany beat London Marathon champion Tigist Tufa of Ethiopia becoming the first back-to-back New York champion since Paula Radcliffe won in 2007 and 2008.
Ethiopia's Aselefech Mergia took second place clocking 2:25:32 with teammate Tufa finishing in third.
"When I took my lead, I said, let me just go," said the 33-year-old Keitany. "If someone is enough strong they can come." She added.
Ethiopia's Aselefech Mergia took second in 2:25:32 with teammate Tufa crossing the line third in 2:25:50.
The top American was Laura Thweatt, who in her marathon debut finished seventh with a time of 2:28.23.
Men top 10 Results
1 | Stanley Biwott | KEN | 2:10:34 |
2 | Geoffery Kamworor | KEN | 2:10:48 |
3 | Lelisa Desisa | ETH | 2:12:10 |
4 | Wilson Kipsang | KEN | 2:12:45 |
5 | Yemane Tsegay | ETH | 2:13:24 |
6 | Yuki Kawauchi | JPN | 2:13:29 |
7 | Meb Keflezighi | USA | 2:13:32 |
8 | Craig Leon | USA | 2:15:16 |
9 | Birhanu Dare Kemal | ETH | 2:15:40 |
10 | Kevin Chelimo | KEN | 2:15:49 |
11 | Andrea Lalli | ITA | 2:17:12 |
Top Women's Results
1 | Mary Keitany | 02:24:25 | KEN |
2 | Aselefech Mergia | 02:25:32 | ETH |
3 | Tigist Tufa | 02:25:50 | ETH |
4 | Sara Moreira | 02:25:53 | POR |
5 | Christelle Daunay | 02:26:57 | FRA |
6 | Priscah Jeptoo | 02:27:03 | KEN |
7 | Laura Thweatt | 02:28:23 | USA |
8 | Jelena Prokopcuka | 02:28:46 | LAT |
9 | Anna Incerti | 02:33:13 | ITA |
10 | Caroline Rotich | 02:33:19 | KEN |
11 | Cassie Fien | 02:38:53 | AUS |